Sybr Green Qpcr Master Mix

Sybr green pcr master mix in a 1 ml vial.

Sybr green qpcr master mix.

Direct detection of pcr product is monitored by measuring the increase in. Azuraquant green and probe fast qpcr master mixes for real time pcr real time pcr primer enzymes. At a point where the fluorescence signal is confidently detected over the background fluorescence a quantification cycle or c q value can be determined. Sybr green pcr master mix and sybr green rt pcr reagents kit user guide 9 1 product information purpose of the kit the sybr green pcr master mix is a convenient premix of the components except primers template and water necessary to perform real time pcr using sybr green i dye.

Qpcr master mix is provided at 2 concentration and can be used at 1 concentration by adding template primer passive. Sybr green for qpcr. Guaranteed minimum shelf life is 60 days exact expiry date printed on product and cofa. The super hot start sybr green qpcr master mix is a ready to use qpcr cocktail.

Power sybr green pcr master mix contains all of the components excluding the template and primers for superior sybr green reagent based real time pcr. It contains a novel taq dna polymerase unique hot start reagents optimized buffer sybr green i dntps pcr enhancer and pcr stabilizer. Sybr green i is a commonly used fluorescent dye that binds double stranded dna molecules by intercalating between the dna bases. Sybr green dye is a fluorescent double stranded dna dsdna binding dye that is used to track the progress of dna amplification in real time pcr experiments.

The added rox reference dye streamlines the reaction setup process. The master mix qrt pcr kits offer you the sensitivity reproducibility and speed you require. Dye based quantitative pcr qpcr uses real time fluorescence of a double stranded dna dsdna binding dye most commonly sybr green i to measure dna amplification during each cycle of a pcr. Sybr green master mix advantages.

Applied biosystems amplitaq gold dna polymerase ld is a hot start pcr enzyme provided in an inactive state allowing for room temperature set up and minimizing non specific amplification. As the pcr reaction proceeds at each round of amplification sybr green dye binds to dsdna as it polymerizes resulting in an increase in the level of fluorescence at the end of each extension step. It is used in quantitative pcr because the fluorescence can be measured at the end of each amplification cycle to determine relatively or absolutely how much dna has been amplified. The qualitative pcr reagents differentiate two fold differences one cycle or one ct equivalence in samples between 5 and 2 5 copy equivalents at 95 efficiency.

Sufficient reagents provided for 40 reactions based on a 50 µl reaction volume.