In rna quantitation assays you use the sybr green pcr master mix in the second step of a two step reverse transcription polymerase chain reaction rt pcr protocol. In a one step rt pcr protocol multiscribe reverse transcriptase and rnase inhibitor are added to the sybr green pcr master mix. Iq and ssofast pcr supermixes have been supporting researchers performing basic real time pcr analysis for years.
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